The role of the NMD factor UPF3B in olfactory sensory neurons

Autor: Kun Tan, Samantha H Jones, Blue B Lake, Jennifer N Chousal, Eleen Y Shum, Lingjuan Zhang, Song Chen, Abhishek Sohni, Shivam Pandya, Richard L Gallo, Kun Zhang, Heidi Cook-Andersen, Miles F Wilkinson
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: eLife, Vol 9 (2020)
Druh dokumentu: article
ISSN: 2050-084X
DOI: 10.7554/eLife.57525
Popis: The UPF3B-dependent branch of the nonsense-mediated RNA decay (NMD) pathway is critical for human cognition. Here, we examined the role of UPF3B in the olfactory system. Single-cell RNA-sequencing (scRNA-seq) analysis demonstrated considerable heterogeneity of olfactory sensory neuron (OSN) cell populations in wild-type (WT) mice, and revealed that UPF3B loss influences specific subsets of these cell populations. UPF3B also regulates the expression of a large cadre of antimicrobial genes in OSNs, and promotes the selection of specific olfactory receptor (Olfr) genes for expression in mature OSNs (mOSNs). RNA-seq and Ribotag analyses identified classes of mRNAs expressed and translated at different levels in WT and Upf3b-null mOSNs. Integrating multiple computational approaches, UPF3B-dependent NMD target transcripts that are candidates to mediate the functions of NMD in mOSNs were identified in vivo. Together, our data provides a valuable resource for the olfactory field and insights into the roles of NMD in vivo.
Databáze: Directory of Open Access Journals