Coupling S-adenosylmethionine-dependent methylation to growth: Design and uses.
Autor: | Hao Luo, Anne Sofie L Hansen, Lei Yang, Konstantin Schneider, Mette Kristensen, Ulla Christensen, Hanne B Christensen, Bin Du, Emre Özdemir, Adam M Feist, Jay D Keasling, Michael K Jensen, Markus J Herrgård, Bernhard O Palsson |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: | |
Zdroj: | PLoS Biology, Vol 17, Iss 3, p e2007050 (2019) |
Druh dokumentu: | article |
ISSN: | 1544-9173 1545-7885 |
DOI: | 10.1371/journal.pbio.2007050 |
Popis: | We present a selection design that couples S-adenosylmethionine-dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of another enzyme category when linked to a methylation pathway in Escherichia coli using adaptive laboratory evolution. We also demonstrate its application for drug discovery using a catechol O-methyltransferase and its inhibitors entacapone and tolcapone. Implementation of this design in Saccharomyces cerevisiae is also demonstrated. |
Databáze: | Directory of Open Access Journals |
Externí odkaz: | |
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