Coupling S-adenosylmethionine-dependent methylation to growth: Design and uses.

Autor:	Hao Luo, Anne Sofie L Hansen, Lei Yang, Konstantin Schneider, Mette Kristensen, Ulla Christensen, Hanne B Christensen, Bin Du, Emre Özdemir, Adam M Feist, Jay D Keasling, Michael K Jensen, Markus J Herrgård, Bernhard O Palsson
Jazyk:	angličtina
Rok vydání:	2019
Předmět:	Biology (General) QH301-705.5
Zdroj:	PLoS Biology, Vol 17, Iss 3, p e2007050 (2019)
Druh dokumentu:	article
ISSN:	1544-9173 1545-7885
DOI:	10.1371/journal.pbio.2007050
Popis:	We present a selection design that couples S-adenosylmethionine-dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of another enzyme category when linked to a methylation pathway in Escherichia coli using adaptive laboratory evolution. We also demonstrate its application for drug discovery using a catechol O-methyltransferase and its inhibitors entacapone and tolcapone. Implementation of this design in Saccharomyces cerevisiae is also demonstrated.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/8a014f6bc453477b97b3c4f4f1ca61cc Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF Plný text ve formátu HTML
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