Longitudinal Study of Mycobacterium avium Subsp. paratuberculosis Antibody Kinetics in Dairy Cattle Using Sera and Milk throughout the Lactation Period

Autor: Md. Shohel Al Faruk, Young-hoon Jung, Tai-young Hur, Sang-suk Lee, Yong-il Cho
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Veterinary Sciences, Vol 7, Iss 3, p 81 (2020)
Druh dokumentu: article
ISSN: 2306-7381
DOI: 10.3390/vetsci7030081
Popis: Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne’s disease in dairy cattle populations around the world. The objective of this study was to evaluate MAP antibody kinetics in serum and milk samples throughout the lactation period in dairy cattle. The samples were collected simultaneously from eight MAP-positive and two healthy MAP-negative (control group) cows. The MAP antibody was detected by using serum and milk ELISA. The serum and milk MAP antibody titers fluctuated between the positive and negative cut-off values in this study. Specifically, cattle with low MAP antibody titer (2 = 0.5358) observed throughout the assessment period. The milk MAP ELISA test had low diagnostic performance in cows with low MAP titer due to its weak correlation (R2 = 0.0198). Finally, this study suggest that the periodic MAP ELISA test is recommended for the application of Johne’s eradication program due to the fluctuating nature of MAP antibody kinetics.
Databáze: Directory of Open Access Journals