| Autor: |
Liuming Xie, Mingyue Shen, Rong Huang, Xuan Liu, Yue Yu, Hanyu Lu, Jianhua Xie |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Food Science and Human Wellness, Vol 12, Iss 5, Pp 1545-1556 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2213-4530 |
| DOI: |
10.1016/j.fshw.2023.02.002 |
| Popis: |
In this study, the antitumor properties and the possible molecular mechanisms of Cyclocarya paliurus polysaccharide (CP) and its phosphorylated derivative (P-CP) on CT-26 mouse colon carcinoma cells were investigated. Results found that CP had high inhibition ratio against CT-26 cells. The flow cytometry results found that CP treatment could cause the intracellular acidification, arrest the cell cycle in the S phase and increase reactive oxygen species generation. Additionally, CP treatment triggered mitochondrial membrane potential depolarization and Ca2+ overloading, and broke down the balance of antioxidant system, Na+/K+-ATPase and Ca2+-ATPase. Further analysis found CP induced cell apoptosis through improving the activities of caspase-3 and caspase-9, and increasing the level of cytochrome C. Furthermore, the comparative study of antitumor effect on CT-26 cells displayed that the phosphorylation enhanced antitumor activities of polysaccharides. These results suggest CP is a potential natural therapeutic agent for colon cancer and phosphorylation represents an effective method of enhancing the antitumor activity of CP. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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