| Autor: |
Kai Cheng, Cao Li, Jiachuan Jin, Xuezhen Qian, Jiayin Guo, Limini Shen, YiChen Dai, Xue Zhang, Zhanwei Li, Yichun Guan, Fei Zhou, Jin Tang, Jun Zhang, Bin Shen, Xin Lou |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Molecular Therapy: Nucleic Acids, Vol 34, Iss , Pp 102028- (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2162-2531 |
| DOI: |
10.1016/j.omtn.2023.09.005 |
| Popis: |
Double-stranded DNA-specific cytidine deaminase (DddA) base editors hold great promise for applications in bio-medical research, medicine, and biotechnology. Strict sequence preference on spacing region presents a challenge for DddA editors to reach their full potential. To overcome this sequence-context constraint, we analyzed a protein dataset and identified a novel DddAtox homolog from Ruminococcus sp. AF17-6 (RsDddA). We engineered RsDddA for mitochondrial base editing in a mammalian cell line and demonstrated RsDddA-derived cytosine base editors (RsDdCBE) offered a broadened NC sequence compatibility and exhibited robust editing efficiency. Moreover, our results suggest the average frequencies of mitochondrial genome-wide off-target editing arising from RsDdCBE are comparable to canonical DdCBE and its variants. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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