Probiotics supplementation reduces cigarette smoke-induced damage in the respiratory micro-architecture of mice
| Autor: | Komal Khan, Hafsa Zaneb, Saima Masood, Saima Ashraf, Mirza Muhammad Usman, Hafiz Faseehur-Rehman, Habibur Rehman |
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| Jazyk: | English<br />Portuguese |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Brazilian Journal of Veterinary Research and Animal Science, Vol 60 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1413-9596 1678-4456 |
| Popis: | This study evaluated the protective efficacy of probiotics supplementation against cigarette smoke-induced lung emphysema, inflammation, and loss of cilia in mice. Probiotics are known to promote mucosal tolerance and mitigate respiratory injuries. Twenty-four adult mice were randomly divided into three groups: control (Ctr), cigarette smoke (CS), and cigarette smoke + probiotics (CS+P). Probiotics were given for 7 days before exposure to smoke in the CS+P group. Tissue samples of the trachea (goblet cell count and index, loss of cilia), lungs (airspace distention), and bronchoalveolar lavage fluid (BALF) were collected and processed. The results showed a significant increase in acidic and neutral goblet cells in the CS group compared to the Ctr and CS+P groups (P < 0.05). Overall, goblet cell number and index were lower in the CS+P group (41.71 ± 5.76, 0.67 ± 0.073) than CS group (56.28 ± 5.34, 1.31 ± 0.28). Inflammatory cells and loss of cilia significantly decreased in mice fed probiotics before exposure to cigarette smoke (P < 0.05). Lung emphysema was also significantly reduced in the CS+P group compared to the CS group (P < 0.05). In conclusion, dietary supplementation of probiotics reduced lung emphysema, inflammatory cells, goblet cell index, and loss of cilia under conditions of cigarette smoke exposure in mice. |
| Databáze: | Directory of Open Access Journals |
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