CELA3B immunostaining is a highly specific marker for acinar cell carcinoma of the pancreas.

Autor: Ria Uhlig, Nina Bröker, Sören Weidemann, Natalia Gorbokon, Anne Menz, Franziska Büscheck, Andreas M Luebke, Devita Putri, Martina Kluth, Claudia Hube-Magg, Andrea Hinsch, Maximilian Lennartz, Viktor Reiswich, Doris Höflmayer, Christoph Fraune, Katharina Möller, Christian Bernreuther, Patrick Lebok, Guido Sauter, Sarah Minner, Stefan Steurer, Eike Burandt, Rainer Krech, David Dum, Andreas Marx, Ronald Simon, Till Krech, Till S Clauditz, Frank Jacobsen
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: PLoS ONE, Vol 18, Iss 6, p e0287528 (2023)
Druh dokumentu: article
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0287528
Popis: Chymotrypsin-like elastase family member 3B (CELA3B, elastase-3B) is a pancreatic enzyme with digestive function in the intestine. Since RNA analyses of normal tissues suggest that CELA3B expression is limited to the pancreas, the potential diagnostic utility of CELA3B immunohistochemistry for the distinction of pancreatic from extrapancreatic cancers and in the distinction of acinar cell carcinoma from ductal adenocarcinoma was assessed. CELA3B expression was successfully analyzed in 13,223 tumor samples from 132 different tumor types and subtypes as well as 8 samples each of 76 different normal tissue types by immunohistochemistry in a tissue microarray format (TMA). In normal tissues, CELA3B immunostaining was only seen in acinar cells and in a fraction of ductal cells of the pancreas as well as on some apical membranes of surface epithelial cells of the intestine. Among tumors, CELA3B immunostaining was seen in 12 of 16 (75%) acinar cell carcinoma of the pancreas including 6 cases with strong staining (37.5%) as well as in 5 of 13,207 other tumors (0.04%). These included 1.2% of 91 adenoid cystic carcinomas, 1.2% of 246 mucoepidermoid carcinomas and 0.8% of 127 acinic cell carcinomas of salivary glands. Our data show a good sensitivity (75%) and a high specificity (99.9%) of CELA3B immunohistochemistry for diagnosing acinar cell carcinoma of the pancreas.
Databáze: Directory of Open Access Journals
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje