Effects of remote ischemic preconditioning on astrocyte proliferation and glial scars after cerebral infarction

Autor: Shi-Min Liu, Xian-Min Cao, Xin-Hui Qu, Wen Cai, Fan Hu, Wen-Feng Cao, Lin-Feng Wu, Xiao-Mu Wu
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: European Journal of Inflammation, Vol 17 (2019)
Druh dokumentu: article
ISSN: 2058-7392
20587392
DOI: 10.1177/2058739219846325
Popis: The aim of this study was to investigate whether remote ischemic preconditioning (RIPC) can promote neurological function recovery after middle cerebral artery occlusion (MCAO) in rats and its possible mechanism. A total of 32 Sprague Dawley (SD) rats were randomly divided into RIPC group (n = 16) and MCAO group (n = 16). In the RIPC group, 1 h before induction of MCAO, the rats received bilateral femoral artery ischemic preconditioning (10 min/time), followed by 10 min of relaxation, and a total of three cycles were carried out. Then, the MCAO-2h model was established. In the MCAO group, the MCAO-2h model was established at 1 h after the separation of bilateral femoral arteries. The modified neurological severity score (mNSS) was assessed. At postmodeling day 7, triphenyltetrazolium chloride (TTC) staining and immunohistochemistry were conducted, and neurological function recovery, infarct size, and the expression levels of glial fibrillary acidic protein (GFAP), synaptophysin (SYN), and neurite outgrowth inhibitor A (Nogo-A) were observed. At postmodeling day 7, the difference in mNSS was statistically significant ( P
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