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Yuting Zhao,1 Xiangzhou Zhang,1 Jie Wang,2 Yong Li,1 Yitong Wu,1 Jisong Liu1 1Department of Plastic Surgery, the Third the People’s Hospital of Bengbu, Bengbu, Anhui, People’s Republic of China; 2Department of Plastic Surgery, Affiliated People’s Hospital of Jiangsu University, Zhenjiang, Jiangsu, People’s Republic of ChinaCorrespondence: Jisong Liu, Department of Plastic Surgery, the Third the People’s Hospital of Bengbu, 38 Shengli Road, Bengbu, Anhui, 233099, People’s Republic of China, Email 1587827999@qq.comBackground: LncRNA zinc finger and SCAN domain containing 16 antisense RNA 1 (ZSCAN16-AS1), a newly identified lncRNA, has been proven to accelerate hepatocellular carcinoma progression. However, the function and molecular mechanism of ZSCAN16-AS1 in melanoma are still unknown.Methods: The level of ZSCAN16-AS1 in melanoma tissues was detected and reported in The Cancer Genome Atlas (TCGA) and GEO#GSE15605. CCK-8, Transwell and flow cytometry assays were used to explore the role of ZSCAN16-AS1 in melanoma cells. Luciferase reporter assays and RNA pull-down assays were used to verify the molecular mechanism of ZSCAN16-AS1.Results: Here, we found that ZSCAN16-AS1 expression was increased in melanoma. We confirmed that ZSCAN16-AS1 promotes the growth and metastasis of melanoma. ZSCAN16-AS1 exerts its pro-tumour role through sponging of miR-503-5p to liberate ADP-ribosylation factor-like protein 2 (ARL2) mRNA transcripts.Conclusion: These results demonstrated the role and molecular mechanism of ZSCAN16-AS1 in the occurrence and development of melanoma. Therefore, ZSCAN16-AS1 may be used as a specific biomarker in the diagnosis and treatment of melanoma patients.Keywords: melanoma, growth and metastasis, ZSCAN16-AS1, miR-503-5p, ARL2 |
