Glucose repression of FLO11 gene expression regulates pellicle formation by a wild pellicle-forming yeast strain isolated from contaminated wine

Autor: Youji Nakagawa, Yukari Arai, Yasuhiro Toda, Hideki Yamamura, Tohru Okuda, Masayuki Hayakawa, Yuzuru Iimura
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Biotechnology & Biotechnological Equipment, Vol 31, Iss 1, Pp 120-127 (2017)
Druh dokumentu: article
ISSN: 1310-2818
1314-3530
13102818
DOI: 10.1080/13102818.2016.1246203
Popis: Some wild strains of Saccharomyces cerevisiae form a pellicle on the surface of contaminated wines during the post-fermentation period of wine making. In this study, we found that both pellicle formation and FLO11 expression by a wild pellicle-forming strain of S. cerevisiae isolated from contaminated wine were repressed in a glucose-containing medium. Substitution of the promoter region of FLO11 in the cells with a constitutive promoter caused derepression of pellicle formation in the glucose-containing medium and wine. These findings indicate that glucose repression of the expression of the FLO11 gene, but not the glucose repression of the expression of other glucose-repressed genes, is responsible for the glucose-dependent regulation of pellicle formation by the wild pellicle-forming yeast strain. Furthermore, we found that the wild pellicle-forming strain had the same deletion of the 111-bp repression sequence in the FLO11 promoter as the flor strains commercially used for making sherry-like wines. Based on the results obtained in this study, a new method that would prevent deleterious pellicle formation in wineries is discussed.
Databáze: Directory of Open Access Journals
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