| Popis: |
Objective To investigate the protective effect of oxygen-glucose deprivation preconditioning (OGDPC) on PC12 cells damaged with oxygen-glucose deprivation/reperfusion (OGD/R) and its underlying mechanism. Methods PC12 cells were randomly divided into Control, OGDPC, OGD/R and OGDPC+OGD/R groups. The morphology and quantity of mitochondria were observed by transmission electron microscopy (TEM) and Mito-tracker staining. The expression of OGDH, PDHA1, COX4 and HSP60 was detected by Western blotting. Mitochondrial membrane potential and ATP production were evaluated by JC-1 and ATP assay kit. TUNEL staining was used to assess cell apoptosis, and translocation of AIF and EnodoG was observed by immunofluorescence staining and TUNEL staining. Results Compared with the OGD/R group, the mitochondrial ultrastructure damage were alleviated, the expression levels of OGDH, PDHA1 and COX4 were enhanced (P < 0.05), that of HSP60 was decreased (P < 0.05), the mitochondrial membrane potential and ATP level were increased (P < 0.05), the cell apoptotic rate was reduced (P < 0.05), and AIF and EndoG were less aggregated to the TUNEL-positive nucleus in OGDPC+OGD/R group (P < 0.05). Conclusion OGDPC can alleviate the injury of PC12 cells caused by OGD/R, and the potential mechanism may be related to improving mitochondrial structure and function, and reducing cell apoptosis via mitochondrial pathway. |
