Expression of Antimicrobial Peptide Genes in the Canine Amniotic Membrane

Autor: Rajit Lohajaroensub, Chenphop Sawangmake, Channarong Rodkhum, Nalinee Tuntivanich
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Veterinary Sciences, Vol 9, Iss 5, p 200 (2022)
Druh dokumentu: article
ISSN: 2306-7381
DOI: 10.3390/vetsci9050200
Popis: The human amniotic membrane has been successfully used in human ocular reconstruction. Several studies have demonstrated its properties, including antimicrobial features. As a result of the restricted availability of human amniotic membrane for veterinary use, canine amniotic membrane has become an attractive alternative. Clinical studies of the application of canine amniotic membrane in animals and the understanding of its biological properties are limited. This study aimed to determine the expression of peptide genes of natural antimicrobials in canine amniotic membrane. Expressions of canine β-defensin 1, 102, and 103, and canine Elafin were determined in healthy puppies by real-time quantitative polymerase chain reaction. Canine β-defensin 1, 103, and Elafin were expressed in all samples, possibly suggesting a role in the innate immune system of normal canine amniotic membrane. Further investigations of protein expression and localization are recommended.
Databáze: Directory of Open Access Journals