| Autor: |
Tim L. Czech, Philipp P. Nelson, Clemens Thölken, Patrick Meyer, Timo Hess, Ho-Ryun Chung, Till Adhikary |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
HardwareX, Vol 18, Iss , Pp e00517- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2468-0672 |
| DOI: |
10.1016/j.ohx.2024.e00517 |
| Popis: |
The advent of single cell technologies resulted in growing demand for microfluidics in the biological sciences. Commercial platforms have remained expensive, inflexible, and non-customizable black boxes. We developed an open source, multichannel, zero-backflow microfluidics device based on syringe pumps controlled by a Raspberry Pi computer. It uses both readily available and 3D-printed parts as well as a custom PCB and is easily serviceable. Moreover, it is fully customizable for various applications. Total cost is under €600. We equipped one channel with a custom Peltier-based temperature controller for precise heating or cooling and a mixer mechanism to prevent sedimentation of the cells within the syringe. Depending on the cells in the sample, heating and cooling can be useful to maintain a beneficial environment or to slow down cellular processes and cell death, respectively. Combined with microfluidics consumables and a microscope, the device is capable of integration into a high quality droplet-based single cell RNA sequencing workflow as shown here. Analysis of a mixture of human and insect cells resulted in a dataset of 17,769 single cells and demonstrates reliable operation and separation. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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