| Autor: |
ZHANG Longyao, ZENG Longjin, ZHENG Linpeng |
| Jazyk: |
čínština |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
陆军军医大学学报, Vol 46, Iss 7, Pp 678-687 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2097-0927 |
| DOI: |
10.16016/j.2097-0927.202312131 |
| Popis: |
Objective To explore the mechanism and effects of Basonuclin 1(BNC1) on the migration and invasion abilities of lung squamous cell carcinoma. Methods Bioinformatics technology was used to analyze the expression patterns and survival impact of BNC1 in patients with lung squamous cell carcinoma in online databases; Tissue microarrays were stained by immunohistochemistry assay and analyzed for BNC1 protein level. Cell lines for lung squamous cell carcinoma with high and low BNC1 expression were screened in the Cancer Cell Line Encyclopedia (CCLE) database. Wound healing experiment and transwell assay were performed on lung squamous cell carcinoma cell models with knockdown and overexpression of BNC1, and the migration and invasion rates of each cell line were detected; Quantitative reverse transcription PCR(RT-qPCR) was used to validate the migration and invasion of 10 highly related genes derived from literature. Western blotting was used to validate the selected regulatory genes. Results Bioinformatics technology found that BNC1 was highly expressed in lung squamous cell carcinoma tissues and was associated with lower survival rate(P=0.047). The expression of BNC1 in lung squamous cell carcinoma was higher than that in paracancerous tissues (P < 0.05). BNC1 had higher expression in the H226 cells and lower expression in the SK-MES1 cells. BNC1 knockdown inhibited the migration and invasion abilities of lung squamous cell carcinoma cells(P < 0.05), and BNC1 overexpression enhanced the migration and invasion abilities of lung squamous cell carcinoma cells(P < 0.05). Screening of BNC1 downstream regulatory molecules at the mRNA level showed that CDH1 was decreased when BNC1 was overexpressed (P < 0.05), and increased when BNC1 was knocked down (P < 0.05). The results of mRNA level were consistent with the results verified by Western blotting (P < 0.05). Conclusion BNC1 may be a gene predicting poor prognosis for lung squamous cell carcinoma patients, and BNC1 may likely to promote cell migration and invasion of lung squamous cell carcinoma by downregulating CDH1 expression. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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