Analytical Methods for the Detection and Quantification of ADCs in Biological Matrices

Autor:	Héloïse Cahuzac, Laurent Devel
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	antibody–drug conjugates pharmacokinetics and biodistribution liquid chromatography coupled to mass spectrometry ligand-binding assays in vivo imaging ex vivo autoradiography Medicine Pharmacy and materia medica RS1-441
Zdroj:	Pharmaceuticals, Vol 13, Iss 12, p 462 (2020)
Druh dokumentu:	article
ISSN:	1424-8247
DOI:	10.3390/ph13120462
Popis:	Understanding pharmacokinetics and biodistribution of antibody–drug conjugates (ADCs) is a one of the critical steps enabling their successful development and optimization. Their complex structure combining large and small molecule characteristics brought out multiple bioanalytical methods to decipher the behavior and fate of both components in vivo. In this respect, these methods must provide insights into different key elements including half-life and blood stability of the construct, premature release of the drug, whole-body biodistribution, and amount of the drug accumulated within the targeted pathological tissues, all of them being directly related to efficacy and safety of the ADC. In this review, we will focus on the main strategies enabling to quantify and characterize ADCs in biological matrices and discuss their associated technical challenges and current limitations.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/8913b6dd16d04f189d6a45e2caf4b99e Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.