In Vitro Anti-Bacterial Activity of Root And Aerial Parts of Scrophularia Striata Bioss on Escherichia Coli, Staphylococcus Aureus and Bacillus Cereus
| Autor: | F Safavi, P Ebrahimi, H Mighani |
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| Jazyk: | perština |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Armaghane Danesh Bimonthly Journal, Vol 18, Iss 8, Pp 603-614 (2013) |
| Druh dokumentu: | article |
| ISSN: | 1728-6506 1728-6514 |
| Popis: | Abstract Background & aim: Due to the side effects of chemical drugs, the replacement of these drugs by natural compounds with antibacterial properties, such as plant extracts, has received much attention. The aim of this study was to evaluate the phytochemical constituents and antimicrobial properties of root and aerial parts of Scrophularia against E. coli, S. aureus and B. cereus in vitro. Methods: In the present study, the antimicrobial activity of different extracts of roots and methanol extract of aerial part the sequential, minimal inhibitory concentrations of bacteria, and minimal bactericidal concentrations of bacteria were determined against the bacteria of E.coli, S. aureus and B. cereus by using both the disk diffusion and the tube dilution method. DMSO was used as the negative control, whereas nalidixic acid and ampicillin were used as the positive control. The collected data was analyzed using the one-way ANOVA test. Results: The results revealed that the root and aerial parts of Scrophularia striata contain flavonoids, glycosides, saponins, tannins and terpenoids. The ethyl acetate and chloroform extracts of root showed no inhibition zone on the bacterial growth. Whereas, the methanolic extract of the root was the most effective against B. cereus with the inhibition zone of the bacterial growth ( 21 mm), followed by MIC and MBC 60 mg/ml 70 mg/ml respectively. The methanol extract of aerial parts had the lowest effect on E.coli Conclusion: The results of the present study revealed that the methanolic extract of root and aerial parts of Scrophularia striata has antibacterial effects, especially against B. cereus. Therefore, further research is needed to identify the different compounds, and also, attainment of new formulation of these components in this plant. Key words: Staphylococcus aureus, Escherichia coli, Bacillus cereus, Antimicrobial, Scrophularia striata. |
| Databáze: | Directory of Open Access Journals |
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