Natural Compounds of Lasia spinosa (L.) Stem Potentiate Antidiabetic Actions by Regulating Diabetes and Diabetes-Related Biochemical and Cellular Indexes

Autor: Md. Mamunur Rashid, Md. Atiar Rahman, Md. Shahidul Islam, Md. Amjad Hossen, A. M. Abu Ahmed, Mirola Afroze, Alaa H. Habib, Manal M. S. Mansoury, Hend F. Alharbi, Reham M. Algheshairy, Walla Alelwani, Afnan M. Alnajeebi, Jitbanjong Tangpong, Srabonti Saha, Alaa Qadhi, Wedad Azhar
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Lasia spinosa
diabetes mellitus
AMPK
PPARγ
Methyl α-
d-galactopyranoside%22">">d-galactopyranoside
methyl α-
d-glucopyranoside%22">">d-glucopyranoside
Medicine
Pharmacy and materia medica
RS1-441
Zdroj: Pharmaceuticals, Vol 15, Iss 12, p 1466 (2022)
Druh dokumentu: article
ISSN: 1424-8247
DOI: 10.3390/ph15121466
Popis: Natural biometabolites of plants have been reported to be useful in chronic diseases including diabetes and associated complications. This research is aimed to investigate how the biometabolites of Lasia spinosa methanol stem (MEXLS) extract ameliorative diabetes and diabetes-related complications. MEXLS was examined for in vitro antioxidant and in vivo antidiabetic effects in a streptozotocin-induced diabetes model, and its chemical profiling was done by gas chromatography-mass spectrometry analysis. The results were verified by histopathological examination and in silico ligand-receptor interaction of characterized natural biometabolites with antidiabetic receptor proteins AMPK (PDB ID: 4CFH); PPARγ (PDB ID: 3G9E); and mammalian α-amylase center (PDB ID: 1PPI). The MEXLS was found to show a remarkable α-amylase inhibition (47.45%), strong antioxidant action, and significant (p < 0.05) decrease in blood glucose level, serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), low-density lipoprotein (LDL), urea, uric acid, creatinine, total cholesterol, triglyceride (TG), liver glycogen, creatinine kinase (CK-MB), and lactate dehydrogenase (LDH) and increase in serum insulin, glucose tolerance, and high-density lipoprotein (HDL). Rat’s pancreas and kidney tissues were found to be partially recovered in histopathological analyses. Methyl α-d-galactopyranoside displayed the highest binding affinity with AMPK (docking score, −5.764), PPARγ (docking score, −5.218), and 1PPI (docking score, −5.615) receptors. Data suggest that the MEXLS may be an exciting source to potentiate antidiabetic activities affirming a cell-line study.
Databáze: Directory of Open Access Journals
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