Ploidy identification of doubled chromosome number plants in Viola × wittrockiana Gams. M 1-generation

Autor:	I. Ajalin, F. Kobza, J. Doležel
Jazyk:	angličtina
Rok vydání:	2002
Předmět:	flow cytometry polyploidisation colchicine hexadecaploids polyploidisation efficiency morphological characteristics viola × wittrockiana gams Agriculture (General) S1-972
Zdroj:	Horticultural Science, Vol 29, Iss 1, Pp 35-40 (2002)
Druh dokumentu:	article
ISSN:	0862-867X 1805-9333
DOI:	10.17221/4468-HORTSCI
Popis:	The aim of this study was to develop a protocol for production of polyploid M1-generation plants of Viola × wittrockiana Gams. Two variants of colchicine treatment were compared for their efficiency. Early detection of novel ploidy levels was achieved by screening of stomata size, leaf index value (leaf blade length/width), and other morphological characteristics of the M1-generation. Secondary screening for novel ploidy levels was performed by flow cytometry (FCM). Hexadecaploid, aneuploid, and mixoploid plants were successfully identified by FCM.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/8889ee8843bc4c5785023d897ef8ab2e Zobrazit plný text záznamu View record in DOAJ