Formation of a Flavin-Linked Peptide

Autor: Masayuki Morikawa, Katsuhito Kino, Takeshi Senda, Masayo Suzuki, Takanobu Kobayashi, Hiroshi Miyazawa
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: Molecules, Vol 19, Iss 7, Pp 9552-9561 (2014)
Druh dokumentu: article
ISSN: 1420-3049
DOI: 10.3390/molecules19079552
Popis: In a previous study, we showed that formylmethylflavin (FMF) can bind to cysteine. In this study, FMF was reacted with native peptides (CG and CKLVFF) containing an N-terminal cysteine. The formation of flavin-CG and flavin-CKLVFF was confirmed using HPLC and ESI-MS. Storage of flavin-CKLVFF in DMSO at −30 °C for 7 days resulted in no detectable deposition. In contrast, flavin-CKLVFF formed deposits when stored in water at −30 °C for 1 day, but no deposit was observed in the aqueous solution of flavin-CKLVFF after 7 days storage in the presence of 0.1% Triton X-100.
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