Autor: |
Masayuki Morikawa, Katsuhito Kino, Takeshi Senda, Masayo Suzuki, Takanobu Kobayashi, Hiroshi Miyazawa |
Jazyk: |
angličtina |
Rok vydání: |
2014 |
Předmět: |
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Zdroj: |
Molecules, Vol 19, Iss 7, Pp 9552-9561 (2014) |
Druh dokumentu: |
article |
ISSN: |
1420-3049 |
DOI: |
10.3390/molecules19079552 |
Popis: |
In a previous study, we showed that formylmethylflavin (FMF) can bind to cysteine. In this study, FMF was reacted with native peptides (CG and CKLVFF) containing an N-terminal cysteine. The formation of flavin-CG and flavin-CKLVFF was confirmed using HPLC and ESI-MS. Storage of flavin-CKLVFF in DMSO at −30 °C for 7 days resulted in no detectable deposition. In contrast, flavin-CKLVFF formed deposits when stored in water at −30 °C for 1 day, but no deposit was observed in the aqueous solution of flavin-CKLVFF after 7 days storage in the presence of 0.1% Triton X-100. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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