Development of Molecular Marker to Detect Citrus Melanose Caused by from Citrus Melanose-like Symptoms
| Autor: | Cheol-Woo Choi, Kyung-Eun Jung, Min-Ju Kim, Su-Hyeon Yoon, Suk-Man Park, Seong-Beom Jin, Jae-Wook Hyun |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | The Plant Pathology Journal, Vol 37, Iss 6, Pp 681-686 (2021) |
| Druh dokumentu: | article |
| ISSN: | 1598-2254 2093-9280 |
| DOI: | 10.5423/PPJ.NT.07.2021.0117 |
| Popis: | It is difficult to distinguish melanose and melanoses-like symptoms with the naked eye because they appear similar. To accurately detect melanose symptoms caused by Diaporthe citri from melanose-like symptoms, we developed PCR-based specific primers Dcitri by aligning the internal transcribed spacer (ITS) region of D. citri with the ITS of Diaporthe cytosporella, Diaporthe foeniculina, Colletotrichum gloeosporioides, Botrytis cinerea, Alternaria citri, and Fusarium oxysporum found on citrus peel. PCR results showed that the specific product was amplified in D. citri but not in other isolates including, C. gloeosporioides, B. cinerea, A. citri, F. oxysporum. In addition, specific products were observed in melanose symptoms caused by D. citri but not in melanose-like symptoms, such as copper-injury, sunscald, damages by yellow tea thrips, and pink citrus rust mite. Using the Dcitri primers developed in this study, it is expected that melanose caused by D. citri could be accurately distinguished from melanose-like symptoms. |
| Databáze: | Directory of Open Access Journals |
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