| Autor: |
S. Tůma, J.K. Izaguirre, M. Bondar, M.M. Marques, P. Fernandes, M.M.R. da Fonseca, M.T. Cesário |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Biotechnology Reports, Vol 27, Iss , Pp e00491- (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2215-017X |
| DOI: |
10.1016/j.btre.2020.e00491 |
| Popis: |
Agar extraction from Gelidium and Gracilaria red seaweed species produces hundred thousand ton of carbohydrate-rich residues annually. Gelidium sesquipedale waste biomass obtained after agar extraction, still contained 44.2 % w/w total carbohydrates (dry-weight basis). These residues were biologically up-graded to poly-3-hydroxybutyrate (P3HB) after saccharification of their carbohydrate fraction to simple sugars. A combined hydrolysis treatment using sulfamic acid followed by enzymatic hydrolysis with cellulases produced a glucose-rich hydrolysate with a negligible content of inhibitors. With this treatment a sugar yield of circa 30 % (g glucose/g biomass) was attained. The algal hydrolysates were assessed as carbon source for the production of P3HB by the halotolerant bacteria Halomonas boliviensis. A cell concentration of 8.3 g L−1 containing 41 % (w/w) of polymer and a yield (YP/S) of 0.16 gpolymer/gglucose were attained in shake flask assays. In this work, cellulose-rich seaweed waste was shown to be an upgradable, sustainable source of carbohydrates. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
