PtHAK5, a candidate for mediating high-affinity K+ uptake in the halophytic grass, Puccinellia tenuiflora

Autor: Haili YANG, Weidan ZHANG, Weiwei CHAI, Wenying WANG, Li GAO, Jing ZHANG, Yongping WANG, Suo-Min WANG
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Frontiers of Agricultural Science and Engineering, Vol 5, Iss 1, Pp 108-117 (2018)
Druh dokumentu: article
ISSN: 2095-7505
DOI: 10.15302/J-FASE-2018200
Popis: Puccinellia tenuiflora is a typical salt-exclu-ding halophytic grass with strong salt-tolerance, which enhances tolerance by restricting Na+ influx as well as having a strong selectivity for K+ over Na+. The HAK5 K+ transporters generally modulate effective K+ acquisition in plants, especially under low K+ condition. In this study, PtHAK5 from P. tenuiflora was isolated by RT-PCR and characterized using yeast complementation. The results showed PtHAK5 consisted of 784 amino acids and shared over 80% homology with the identified high-affinity K+ transporter HAK5 from other higher plants. The expression of PtHAK5 rescued the K+-uptake-defective phenotype of yeast strain CY162. In conclusion, PtHAK5 is a candidate for mediating high-affinity K+ uptake under low K+ conditions.
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