Characterization of Two Novel Rumen-Derived Exo-Polygalacturonases: Catalysis and Molecular Simulations

Autor: Qian Deng, Xiaobao Sun, Deying Gao, Yuting Wang, Yu Liu, Nuo Li, Zhengguang Wang, Mingqi Liu, Jiakun Wang, Qian Wang
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Microorganisms, Vol 11, Iss 3, p 760 (2023)
Druh dokumentu: article
ISSN: 2076-2607
DOI: 10.3390/microorganisms11030760
Popis: Pectinases are a series of enzymes that degrade pectin and have been used extensively in the food, feed, and textile industries. The ruminant animal microbiome is an excellent source for mining novel pectinases. Two polygalacturonase genes, IDSPga28-4 and IDSPga28-16, from rumen fluid cDNA, were cloned and heterologously expressed. Recombinant IDSPGA28-4 and IDSPGA28-16 were stable from pH 4.0 to 6.0, with activities of 31.2 ± 1.5 and 330.4 ± 12.4 U/mg, respectively, against polygalacturonic acid. Hydrolysis product analysis and molecular dynamics simulation revealed that IDSPGA28-4 was a typical processive exo-polygalacturonase and cleaved galacturonic acid monomers from polygalacturonic acid. IDSPGA28-16 cleaved galacturonic acid only from substrates with a degree of polymerization greater than two, suggesting a unique mode of action. IDSPGA28-4 increased the light transmittance of grape juice from 1.6 to 36.3%, and IDSPGA28-16 increased the light transmittance of apple juice from 1.9 to 60.6%, indicating potential application in the beverage industry, particularly for fruit juice clarification.
Databáze: Directory of Open Access Journals
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