Effects of fibroblast growth factor-2 on cell proliferation of cementoblasts
| Autor: | Hui-Chieh Yu, Fu-Mei Huang, Shiuan-Shinn Lee, Cheng-Chia Yu, Yu-Chao Chang |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Journal of Dental Sciences, Vol 11, Iss 4, Pp 463-467 (2016) |
| Druh dokumentu: | article |
| ISSN: | 1991-7902 84054891 |
| DOI: | 10.1016/j.jds.2016.06.007 |
| Popis: | Background/purpose: Fibroblast growth factor (FGF)-2 is known as a signaling molecule that induces tissue regeneration. Little is known about the effect of FGF-2 on cementoblasts for periodontal and periapical regeneration. The aim of this study was to investigate the effects of FGF-2 on murine immortalized cementoblast cell line (OCCM.30). Materials and methods: Cell growth and proliferation was judged by using alamar blue reduction assay. Flow cytometry analysis was used to evaluate Stro-1 positive cells expression with or without FGF-2. Western blot was used to evaluate the expression of phosphorylated serine–threonine kinase Akt (p-Akt) and extracellular signal-regulated protein kinase (p-ERK) in cementoblasts. Results: FGF-2 was found to increase cell growth in a dose-dependent manner (P |
| Databáze: | Directory of Open Access Journals |
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