Plant transcriptome analysis reveals specific molecular interactions between alfalfa and its rhizobial symbionts below the species level

Autor: Wenjuan Kang, Zhehao Jiang, Yonggang Chen, Fang Wu, Chang Liu, Haifang Wang, Shangli Shi, Xue-Xian Zhang
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: BMC Plant Biology, Vol 20, Iss 1, Pp 1-19 (2020)
Druh dokumentu: article
ISSN: 1471-2229
DOI: 10.1186/s12870-020-02503-3
Popis: Abstract Background Leguminous plants alter patterns of gene expression in response to symbiotic colonization and infection by their cognate rhizobial bacteria, but the extent of the transcriptomic response has rarely been examined below the species level. Here we describe the identification of 12 rhizobial biotypes of Ensifer meliloti, which form nitrogen-fixing nodules in the roots of alfalfa (Medicago sativa L.), followed by a comparative RNA-seq analysis of four alfalfa cultivars each inoculated with two E. meliloti strains varying in symbiotic performance and phylogenetic relatedness. Results Rhizobial biotypes were identified on the basis of their symbiotic performance, particularly shoot dry weight. Differentially expressed genes (DEGs) and metabolic pathways were determined by comparing the RNA-seq data with that of the uninoculated control plant. Significant differences were found between DEGs generated in each cultivar with the inoculation of two rhizobial strains in comparison (P
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