CD38 Drives Progress of Osteoarthritis by Affecting Cartilage Homeostasis

Autor: Jin‐jin Ma, Jun Ying, Jin‐yu Wang, Tao‐tao Xu, Han‐ting Xia, Hong‐ting Jin, Lu‐wei Xiao, Wen‐jun Shang, Wei‐qian Wang, Jian‐yin Feng
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Orthopaedic Surgery, Vol 14, Iss 5, Pp 946-954 (2022)
Druh dokumentu: article
ISSN: 1757-7861
1757-7853
DOI: 10.1111/os.13258
Popis: Objective To observe expression of CD38, a key modulator of nicotinamide dinucleotide (NAD+) metabolism in mice with knee osteoarthritis, and protective effect of CD38 inhibition during the osteoarthritis (OA) development. Method The destabilization of the medial meniscus (DMM) model was performed in mice to mimic the process of OA. Immunofluorescence of CD38 was performed to evaluate its response during the OA process. Limb bud‐derived mesenchymal cells were isolated for micromass culture. 100 nM or 1 μM CD38 inhibitor (78c) treatment for 14 days and CD38 sgRNA infection were then used to explore the effects of chondrogenic differentiation via Alcian blue staining. The expressions of chondrogenic markers were detected using RT‐PCR and Western blot. To explore the protective effect of CD38 inhibitor on cartilage degradation during OA in vivo, a CD38 inhibitor was injected into the knee joint after DMM operations. Micro‐CT analysis and Safranin O‐fast green staining were used to evaluate subchondral bone micro‐architecture changes and cartilage degeneration. Results Compared to the control group, the CD38 expression in superficial cartilage was obviously increased in DMM group (P
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