Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)
| Autor: | Gabriela Kinue Watase Kunii, Rossana Falcone, Leandro da Costa Clementino, João Aristeu da Rosa, Juliana Damieli Nascimento, Tiago Belintani, Jader de Oliveira, Aline Rimoldi Ribeiro |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Revista da Sociedade Brasileira de Medicina Tropical, Vol 55 (2022) |
| Druh dokumentu: | article |
| ISSN: | 1678-9849 0037-8682 |
| DOI: | 10.1590/0037-8682-0521-2021 |
| Popis: | ABSTRACT Background: Trypanosoma cruzi presents great variability in morphology, virulence, pathogenicity, avoidance of the host immune system, and antigenic constitution, associated with different clinical manifestations of the disease. Methods: Two strains of T. cruzi were cultivated in liver infusion tryptose to determine growth kinetics, morphometry and molecular characterization using restriction fragment length polymorphism polymerase chain reaction. Results: The biological parameters showed sharp growth by the 7th day. Morphologically, both strains showed short and thin forms and were classified as Group I. Conclusion: Group TcI presents cardiac manifestations and T. sherlocki is adapting to the home environment, requiring attention to future problems. |
| Databáze: | Directory of Open Access Journals |
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