IgG Antibody 3D Structures and Dynamics
| Autor: | Jacob White Jay, Brinkley Bray, Yaozhi Qi, Eseosaserea Igbinigie, Hao Wu, Jinping Li, Gang Ren |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
single molecule 3D image
individual-particle 3D image antibody structure bispecific antibody electron tomography individual-particle electron tomography IPET 3D structure of IgG antibody dynamics antibody engineering homodimer antibody structure of bispecific IgG1 Immunologic diseases. Allergy RC581-607 |
| Zdroj: | Antibodies, Vol 7, Iss 2, p 18 (2018) |
| Druh dokumentu: | article |
| ISSN: | 2073-4468 |
| DOI: | 10.3390/antib7020018 |
| Popis: | Antibodies are vital for human health because of their ability to function as nature’s drugs by protecting the body from infection. In recent decades, antibodies have been used as pharmaceutics for targeted therapy in patients with cancer, autoimmune diseases, and cardiovascular diseases. Capturing the dynamic structure of antibodies and characterizing antibody fluctuation is critical for gaining a deeper understanding of their structural characteristics and for improving drug development. Current techniques for studying three-dimensional (3D) structural heterogeneity and variability of proteins have limitations in ascertaining the dynamic structural behavior of antibodies and antibody-antigen complexes. Here, we review current techniques used to study antibody structures with a focus on the recently developed individual-particle electron tomography (IPET) technique. IPET, as a particle-by-particle methodology for 3D structural characterization, has shown advantages in studying structural variety and conformational changes of antibodies, providing direct imaging data for biomolecular engineering to improve development and clinical application of synthetic antibodies. |
| Databáze: | Directory of Open Access Journals |
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