Effect of Serum Starvation and Full Confluence on Cell Cycle Synchronization and Apoptosis of Goat Dermal Fibroblast

Autor:	Azam Dalman, Poopak Eftekhari Yazdi, Mojtaba Rezazadeh Valojerdi, Abdolhossein Shahverdi, Hamid Gourabi, Rahman Fakheri, Ehsan Janzamin, Fatemeh Sadeghian
Jazyk:	angličtina
Rok vydání:	2009
Předmět:	Cell Cycle Synchronization Serum Starve Apoptosis Fibroblast Medicine Science
Zdroj:	Cell Journal, Vol 11, Iss 2, Pp 212-219 (2009)
Druh dokumentu:	article
ISSN:	2228-5806 2228-5814
Popis:	Objective: We examine the effects of serum starvation, culture to confluence, and full confluenceon cell cycle characteristics and apoptosis of goat dermal fibroblast cells.Materials and Methods: Fibroblast cells were obtained from the ear of a female goat, 1.5years of age. The following experimental groups were analyzed for fibroblast cells: 1. cellsconfluent for 72h, 2. cells starved for 48 and 72h.Results: Analysis of cell cycle distribution by flow cytometry showed that 4.56, 51.88 ofnormal cycling cells were at the G0, G1 phases, respectively. Serum starvation for 48 and72h arrested cells at the G0/G1 phase (p
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/877f8e01828345eaae3564790cbd1487 Zobrazit plný text záznamu View record in DOAJ