| Autor: |
Sayoko Ito‐Harashima, Mai Matsuura, Eiji Takada, Masanobu Kawanishi, Yoshiaki Nakagawa, Takashi Yagi |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
FEBS Open Bio, Vol 11, Iss 10, Pp 2774-2783 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2211-5463 |
| DOI: |
10.1002/2211-5463.13277 |
| Popis: |
Juvenile hormones (JHs) are sesquiterpenoids that play important roles in the regulation of growth, metamorphosis, and reproduction in insects. Synthetic JH agonists (JHAs) have been used as insecticides and are categorized as a class of insect growth regulators (IGRs). Natural JHs and synthetic JHAs bind to the JH receptor methoprene‐tolerant (Met), which forms a functional JH‐receptor complex with steroid receptor coactivators, such as Drosophila melanogaster Taiman (Tai). The ligand‐bound Met–Tai complex induces the transcription of JH response genes by binding to specific DNA elements referred to as JH response elements (JHREs). In the present study, we established a reporter gene assay (RGA) for detecting natural JHs and synthetic JHAs in a yeast strain expressing D. melanogaster Met and Tai. The yeast RGA system detected various juvenoid ligands in a dose‐dependent manner. The rank order of the ligand potencies of the juvenoids examined in the yeast RGA linearly correlated with those of RGAs for Met–Tai established in mammalian and insect cells. Our new yeast RGA is rapid, easy to handle, cost‐effective, and valuable for screening novel JHAs. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
Plný text