Effects of HMGB1/TLR4 on secretion IL-10 and VEGF in human jaw bone-marrow mesenchymal stem cells
| Autor: | Jingjing Kong, Wei Cheng, Lianzhen Chang, Jingyi Yu, Ronglin Wang, Jianli Xie |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Journal of Applied Oral Science, Vol 32 (2024) |
| Druh dokumentu: | article |
| ISSN: | 1678-7765 1678-7757 |
| DOI: | 10.1590/1678-7757-2023-0304 |
| Popis: | Abstract Objective: We aimed to investigate the regulatory effects of HMGB1/TLR4 signaling pathway on the expression of IL-10 and VEGF in human bone marrow mesenchymal stem cells. Methodology: Human JBMSCs were isolated and cultured. Then, HMGB1 was added into the JBMSCs culture medium, and the protein and mRNA expression levels of IL-10 and VEGF were assessed. Moreover, cells were pretreated with a specific TLR4 inhibitor (TAK-242), and the expression changes of IL-10 and VEGF were compared. Results: Compared with the control group, exposure to HMGB1 in human JBMSCs up-regulated TLR4, IL-10, and VEGF secretion at both protein and mRNA levels (P |
| Databáze: | Directory of Open Access Journals |
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