Differential expression of cytokines and receptor expression during anoxic growth

Autor:	Balbina J. Plotkin, Ira M. Sigar, Julie A. Swartzendruber, Amber Kaminski, James Davis
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	Anoxia Anaerobic Cytokine IL-6 IL-8 IL-11 Medicine Biology (General) QH301-705.5 Science (General) Q1-390
Zdroj:	BMC Research Notes, Vol 11, Iss 1, Pp 1-6 (2018)
Druh dokumentu:	article
ISSN:	1756-0500
DOI:	10.1186/s13104-018-3520-5
Popis:	Abstract Objective Cell density in tumor cell three dimensional (3D) cultures affects secretome expression of components. A microenvironment characteristic shared by high-density 3D cell culture and in vivo tumor masses is poor oxygenation, with anoxia being a natural cell state in tumor centers. Until recently, the ability to study anoxia-adapted cell physiology was not possible. Using a newly-developed methodology, anoxic HeLa cell secretome expression was measured. Results Anoxic HeLa cell cytokine levels after 3 days’ (hypoxia inducible factor, HIF1 positive) and 10 days’ growth (HIF1 negative; anaerobic respiration) were significantly (p
Databáze:	Directory of Open Access Journals
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