| Autor: |
Eliane Avany Malveira Araújo, Geisy Rebouças Lima, Luciana Aleixo dos Santos de Melo, Leilane Bentes de Sousa, Marne Carvalho de Vasconcellos, Nikeila Chacon de Oliveira Conde, Carina Toda, Simone Assayag Hanan, Ary de Oliveira Alves Filho, Maria Fulgência Costa Lima Bandeira |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Advances in Pharmacological and Pharmaceutical Sciences, Vol 2021 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2633-4690 |
| DOI: |
10.1155/2021/8840570 |
| Popis: |
Aim. This study sets out to evaluate the antiproteolytic activity of copaiba oil-based emulsion at the resin/dentin adhesive interface union formed with conventional and self-etching adhesives systems. Methods. At in situ zymography, 30 teeth were sectioned 2 mm below the enamel-dentin junction; a smear layer was standardized and subdivided into four groups. Gelatin conjugated with fluorescein was used and taken to the fluorescence microscope for evaluation. In cytotoxicity, the Trypan Blue method was used at four different time points. The tested groups were (G1) control with distilled water; (G2) 2% chlorhexidine (CLX); (G3) emulsion based on copaiba oil (EC) 10% + X; (G4) 10% EC + Y; and (G5) EC 10% alkaline. The zymographic assay used the same groups described, but in 30 seconds and 10 and 20 minutes. HT1080 cells were incubated and submitted to electrophoresis. The gel was analyzed using ImageJ software. Mann–Whitney and Kruskal–Wallis tests were used in the statistical analysis (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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