Comprehensive analysis of differently expression mRNA and non-coding RNAs, and their regulatory mechanisms on relationship in thiram-induced tibial dyschondroplasia in chicken

Autor: Yuxiang Lu, Hengyong Xu, Yuru Jiang, Zhi Hu, Ranran Du, Xiaoling Zhao, Yaofu Tian, Qing Zhu, Yao Zhang, Yiping Liu, Yan Wang
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Ecotoxicology and Environmental Safety, Vol 242, Iss , Pp 113924- (2022)
Druh dokumentu: article
ISSN: 0147-6513
DOI: 10.1016/j.ecoenv.2022.113924
Popis: Thiram pollution is one of the main causes of tibial dyschondroplasia (TD) induced by feed sources. Several studies have speculated that miRNA, circRNA and lncRNA may have significant impact on the development of TD, however, the specific mRNAs and noncoding RNAs and their respective regulatory mechanisms and functions in the development of TD have not been explored. Therefore, in this present study, we screened the differentially expressed mRNA, miRNA, circRNA and lncRNA by whole-transcriptome sequencing (RNA-seq) and differentially expressed genes (DEGs) enrichment, as well as constructed the interaction network among the mRNA-miRNA, mRNA-lncRNA and mRNA-miRNA-circRNA. The sequencing results were verified by fluorescence real-time quantitative PCR (RT-qPCR). The results obtained in this study, revealed that the cells were atrophied and disordered in the TD group, and the expression of BMP6, TGF-β and VEGF were significantly reduced. A total of 141 mRNAs, 10 miRNAs, 23 lncRNAs and 35 circRNAs of DEGs were obtained (p
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