Influence of Glyphaea brevis twig extract on nucleus, tight junctions and expression of inhibin-β, stem cell factor, and androgen binding protein in TM4 Sertoli cells
| Autor: | Janet Olayemi Olugbodi, Oladipupo David, Oluwafemi Adeleke Ojo, Afolabi Clement Akinmoladun |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Asian Pacific Journal of Reproduction, Vol 8, Iss 4, Pp 157-166 (2019) |
| Druh dokumentu: | article |
| ISSN: | 2305-0500 2305-0519 |
| DOI: | 10.4103/2305-0500.262832 |
| Popis: | Objective: To examine the influence of Glyphaea (G.) brevis twig extract on the mitochondrial dehydrogenase activity, integrity of the tight junctions between adjacent cells, mitochondria, apoptosis, nucleus and expression of inhibin-ß, stem cell factor, and androgen binding protein in TM4 Sertoli cells. Methods: TM4 cell line was used in this study as it exhibited properties similar to the Sertoli cells. TM4 Sertoli cells were exposed to G. brevis twig extract (0.1, 1.0, 10.0, 100.0, or 1 000.0μg/mL) for 24, 48 and 72 h. Parameters studied included cell viability [3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay], mitochondrial membrane potential (tetra methyl rhodamine ethyl ester dye), transepithelial electrical resistance, apoptosis (Annexin V Alexa Fluor®488/propidium iodide assay) and mRNA expression (quantitative reverse transcription polymerase chain reaction). Results: G. brevis twig extract had no cytotoxic impact on cell viability, thus, considerably increasing the activity of mitochondrial dehydrogenase enzyme after 24 and 72 h exposure. Transepithelial electrical resistance values revealed substantial (P |
| Databáze: | Directory of Open Access Journals |
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