Autor: |
Hong Chen, Cheng Wei, Zhiyu Lin, Jinfen Pei, Hao Pan, Haixing Li |
Jazyk: |
angličtina |
Rok vydání: |
2024 |
Předmět: |
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Zdroj: |
STAR Protocols, Vol 5, Iss 1, Pp 102864- (2024) |
Druh dokumentu: |
article |
ISSN: |
2666-1667 |
DOI: |
10.1016/j.xpro.2024.102864 |
Popis: |
Summary: Here, we describe a protocol based on semi-site-specific primer PCR (3SP-PCR) to access unknown flanking DNA sequences. We specify the guidelines for designing primers for 3SP-PCR. We also describe experimental procedures for the 3SP-PCR, along with PCR product purification and subsequent sequencing and analysis.For complete details on the use and execution of this protocol, please refer to Wei et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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