| Autor: |
Ralf Wenz, Emily Conibear, Laurence Bugeon, Maggie Dallman |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
F1000Research, Vol 9 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2046-1402 |
| DOI: |
10.12688/f1000research.22464.1 |
| Popis: |
The availability of transparent zebrafish mutants (either TraNac: trab6/b6; nacw2/w2 or casper: roya9/a9; nacw2/w2) for live imaging studies together with the ease of generating transgenic lines are two of the strengths of the zebrafish model organism. The fact that transparent casper (roya9/a9;nacw2/w2) and silver nacre (nacw2/w2) mutants are indistinguishable by eye at early stages (1-5 days post-fertilization; dpf) means many fish must be raised and later culled if they are not transparent. To identify translucent mutants early and easily at the early larval stage (≤5 dpf) before they are classified as protected animals, we developed a simple screening method using standard fluorescence microscopy. We estimate that this procedure could annually save 60,000 animals worldwide. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
