Numerical methods and image processing techniques for melissopalynological honey analysis

Autor: Zdeňka Javůrková, Matej Pospiech, Simona Ljasovská, Pavel Hrabec, Bohuslava Tremlová
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Potravinarstvo, Vol 15, Pp 58-65 (2021)
Druh dokumentu: article
ISSN: 1337-0960
DOI: 10.5219/1517
Popis: Pollen analysis is a method used for verification of the botanical and geographical honey origin. Currently, much effort is being made to introduce automated systems with the use of image analysis programs. The automatic analysis is impeded by the insufficient depth of field of objects when using a light microscope, however, this can be avoided by using image reconstruction from images obtained from different focal planes. In this method, testing was performed on the normal focus (NF) and extended-depth-of-focus (EDF) images. These two methods were compared and statistically evaluated. The number of pollen grains and selected morphometric characteristics were compared. For EDF images, a higher number of pollen grains was obtained for the analysis, and except for the length/width ratio, a statistically significant difference was observed in the characteristics of pollen grains between the compared NF and EDF methods.
Databáze: Directory of Open Access Journals