| Autor: |
Stephen C. Zambrzycki, Samaneh Saberi, Rachel Biggs, Najmeh Eskandari, Davide Delisi, Harrison Taylor, Anand S. Mehta, Richard R. Drake, Saverio Gentile, Amy D. Bradshaw, Michael Ostrowski, Peggi M. Angel |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Matrix Biology Plus, Vol 24, Iss , Pp 100161- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2590-0285 |
| DOI: |
10.1016/j.mbplus.2024.100161 |
| Popis: |
While numerous approaches have been reported towards understanding single cell regulation, there is limited understanding of single cell production of extracellular matrix phenotypes. Collagens are major proteins of the extracellular microenvironment extensively used in basic cell culture, tissue engineering, and biomedical applications. However, identifying compositional regulation of collagen remains challenging. Here, we report the development of In vitro ExtraCellular Matrix Mass Spectrometry Imaging (ivECM-MSI) as a tool to rapidly and simultaneously define collagen subtypes from coatings and basic cell culture applications. The tool uses the mass spectrometry imaging platform with reference libraries to produce visual and numerical data types. The method is highly integrated with basic in vitro strategies as it may be used with conventional cell chambers on minimal numbers of cells and with minimal changes to biological experiments. Applications tested include semi-quantitation of collagen composition in culture coatings, time course collagen deposition, deposition altered by gene knockout, and changes induced by drug treatment. This approach provides new access to proteomic information on how cell types respond to and change the extracellular microenvironment and provides a holistic understanding of both the cell and extracellular response. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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