Macrobrachium amazonicum: an alternative for microbiological monitoring of aquatic environments in Brazil

Autor: Raimunda Sâmia Nogueira Brilhante, Manoel de Araújo Neto Paiva, Célia Maria de Souza Sampaio, Carlos Eduardo Cordeiro Teixeira, Joyce Fonteles Ribeiro, Débora de Souza Collares Maia Castelo-Branco, Tereza de Jesus Pinheiro Gomes Bandeira, André Jalles Monteiro, Rossana de Aguiar Cordeiro, José Júlio Costa Sidrim, Frederico Ozanan Barros Monteiro, José Luciano Bezerra Moreira, Marcos Fábio Gadelha Rocha
Jazyk: English<br />Portuguese
Rok vydání: 2014
Předmět:
Zdroj: Ciência Rural, Vol 44, Iss 11, Pp 2029-2034 (2014)
Druh dokumentu: article
ISSN: 1678-4596
0103-8478
DOI: 10.1590/0103-8478cr20140016
Popis: This study aimed to evaluate the role of the Amazon River prawn, Macrobrachium amazonicum, as carrier of Candida spp., by analyzing the correlation between Candida spp. from these prawns and their environment (surface water and sediment), through M13-PCR fingerprinting and RAPD-PCR. For this purpose, 27 strains of Candida spp. were evaluated. These strains were recovered from the gastrointestinal tract of adult M. amazonicum (7/27) from Catú Lake, Ceará State, Brazil and from the aquatic environment (surface water and sediment) of this lake (20/27). Molecular comparison between the strains from prawns and the aquatic environment was conducted by M13-PCR fingerprinting and RAPD-PCR, utilizing the primers M13 and OPQ16, respectively. The molecular analysis revealed similarities between the band patterns of eight Candida isolates with the primer M13 and 11 isolates with the primer OPQ16, indicating that the same strains are present in the digestive tract of M. amazonicum and in the aquatic environment where these prawns inhabit. Therefore, these prawns can be used as sentinels for environmental monitoring through the recovery of Candida spp. from the aquatic environment in their gastrointestinal tract
Databáze: Directory of Open Access Journals
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