Potential for in vitro mesoderm differentiation of Wharton's jelly cells from ovine umbilical cord isolated in different culture media
| Autor: | Ronaldo P. Dias, Maria F.S. Teixeira, Edmara C. Costa, Anderson C. Farias, Dalva A.A. Azevedo, Tereza D.F. Aguiar, Mariana A. Pinheiro |
|---|---|
| Jazyk: | English<br />Portuguese |
| Předmět: | |
| Zdroj: | Pesquisa Veterinária Brasileira, Vol 36, Iss suppl 1, Pp 79-88 |
| Druh dokumentu: | article |
| ISSN: | 1678-5150 0100-736X |
| DOI: | 10.1590/S0100-736X2016001300012 |
| Popis: | Abstract: The mammalian Wharton's jelly of umbilical cord (WJUC) is a promising source of multipotent cells, providing advantages due to ethical implications, ease of collection and the absence of teratomas in pre-clinical trials. Ovine multipotent cells have already been isolated from various tissues, however there are no reports using umbilical cords in this species. This study aimed to investigate the best medium to transport the umbilical cord, to isolate and maintain ovine WJUC cells and to compare in vitro growth and mesodermal differentiation potential. Eight ovine umbilical cords were obtained during parturition, sectioned and transported in six different media: MEM, low glucose DMEM, M199, RPMI 1640, PBS and saline. For each transportation medium, four culture media were used and the tissue was explanted in 24-well plates and cultured in MEM, low glucose DMEM, M199 and RPMI 1640, all with 10% FBS. Every experiment was conducted with low-passage (P2), investigating MTT viability during four days and adipogenic, chondrogenic and osteogenesis differentiation was induced in vitro. The most effective transport medium (p |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|