Label-free in-vivo classification and tracking of red blood cells and platelets using Dynamic-YOLOv4 network

Autor: Caizhong Guan, Bin He, Hongting Zhang, Shangpan Yang, Yang Xu, Honglian Xiong, Yaguang Zeng, Mingyi Wang, Xunbin Wei
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Journal of Innovative Optical Health Sciences, Vol 17, Iss 05 (2024)
Druh dokumentu: article
ISSN: 17935458
1793-7205
1793-5458
DOI: 10.1142/S1793545824500093
Popis: In-vivo flow cytometry is a noninvasive real-time diagnostic technique that facilitates continuous monitoring of cells without perturbing their natural biological environment, which renders it a valuable tool for both scientific research and clinical applications. However, the conventional approach for improving classification accuracy often involves labeling cells with fluorescence, which can lead to potential phototoxicity. This study proposes a label-free in-vivo flow cytometry technique, called dynamic YOLOv4 (D-YOLOv4), which improves classification accuracy by integrating absorption intensity fluctuation modulation (AIFM) into YOLOv4 to demodulate the temporal features of moving red blood cells (RBCs) and platelets. Using zebrafish as an experimental model, the D-YOLOv4 method achieved average precisions (APs) of 0.90 for RBCs and 0.64 for thrombocytes (similar to platelets in mammals), resulting in an overall AP of 0.77. These scores notably surpass those attained by alternative network models, thereby demonstrating that the combination of physical models with neural networks provides an innovative approach toward developing label-free in-vivo flow cytometry, which holds promise for diverse in-vivo cell classification applications.
Databáze: Directory of Open Access Journals
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