| Autor: |
Shifeng LIU, Wenjing DONG, Lan YANG, Jiali ZHOU, Dongbo LIU |
| Jazyk: |
čínština |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Shipin gongye ke-ji, Vol 44, Iss 23, Pp 314-321 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1002-0306 |
| DOI: |
10.13386/j.issn1002-0306.2023020035 |
| Popis: |
Objective: To investigate the effects of Ganoderma lucidum polysaccharides and its flora metabolites on the insulin resistance status of HepG2 cells and its mechanisms. Methods: Insulin resistant HepG2 (IR-HepG2) model was established with the combination of insulin (10−3 µmol/L) and dexamethasone (10 µmol/L). The cytotoxicity of Ganoderma lucidum polysaccharides (GLP) and Ganoderma lucidum polysaccharide flora metabolite (GLP-F) was evaluated using the CCK-8 method. The effects of GLP and GLP-F on glucose consumption and glycogen synthesis in IR-HepG2 cells were evaluated using the glucose kit and glycogen kit methods. Western blot assay was used to detect the effects of GLP and GLP-F on the phosphorylation or expression of IRS-1, AKT, GSK-3β, GLUT2, and PEPCK, key proteins in the insulin signaling cascade in IR-HepG2 cells. Results: Both GLP and GLP-F significantly increased glucose uptake and glycogen synthesis in IR-HepG2 cells (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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