Autonomous replication sequences from the Amaranthus palmeri eccDNA replicon enable replication in yeast

Autor:	William T. Molin, Allison Yaguchi, Mark Blenner, Christopher A. Saski
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	eccDNA replicon Autonomous replication EACS DNA bending Medicine Biology (General) QH301-705.5 Science (General) Q1-390
Zdroj:	BMC Research Notes, Vol 13, Iss 1, Pp 1-6 (2020)
Druh dokumentu:	article
ISSN:	1756-0500
DOI:	10.1186/s13104-020-05169-0
Popis:	Abstract Objective The objective of the research presented here was to determine whether autonomous replication sequences (ARS) discovered in the eccDNA replicon of glyphosate resistant Amaranthus palmeri enable self-replication in a yeast system. Results Sequence analysis of the eccDNA replicon revealed a region of sharp changes in A + T/G + C content with characteristic bending indicative of an autonomous replication sequence. Further sequence analysis revealed an extended autonomous replication sequence (EACS) in close proximity to multiple DNA unwinding element (DUE) sequences. This region of the eccDNA replicon enabled autonomous replication of an ARS-less yeast plasmid.
Databáze:	Directory of Open Access Journals
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