| Autor: |
Karen Araújo Magalhães, Kamily Fagundes Pussi, Hélton Krisman de Araújo, Silvia Barbosa do Carmo, Elisabete Friozi, Lidiane Schultz Branquinho, Manoel Sebastião da Costa Lima Junior, Herintha Coeto Neitzke-Abreu |
| Jazyk: |
English<br />Spanish; Castilian<br />Portuguese |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Revista Brasileira de Parasitologia Veterinária, Vol 30, Iss 3 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
1984-2961 |
| DOI: |
10.1590/s1984-29612021061 |
| Popis: |
Abstract The dog is the main domestic reservoir of Leishmania and font of infection for the vector, constituting an important host for the transmission of the parasite to humans. Non-invasive collection of swab samples for leishmaniasis diagnosis has been a promising alternative. This study analyzed the positivity of polymerase chain reaction (PCR) for the diagnosis of canine leishmaniasis in conjunctiva samples. DNA extraction was performed using SDS 20% and PCR was performed using 13A/13B primers that amplify 120-bp of Leishmania kDNA. Of the 77 dogs analyzed, 50 (64.93%) had ocular changes: 25 (32.47%) dogs had periocular lesion, 41 (53.25%) dogs had purulent eye discharge, and 17 (22.08%) dogs had both signals. PCR was positive in 35 dogs (45.45%), and there was no significant difference between dogs with and without ocular signals (p=0.4074). PCR positivity was significant higher in dogs without periocular injury (p=0.0018). Conjunctive PCR, a less invasive, fast, and painless collection technique, is indicated to complement the diagnosis, especially in dogs without periocular injury, independent of the presence of purulent eye discharge. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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