Evaluation of Genetic and Environmental Risk Factors for Deep Vein Thrombosis in Sudanese

Autor: Nadir Ahmed Ibrahim, Fathelrahman M Hassan Gameel, Mahmoud Mohamed Elgari, Sana Eltahir Abdalla
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Clinical and Diagnostic Research, Vol 12, Iss 9, Pp GC07-GC10 (2018)
Druh dokumentu: article
ISSN: 2249-782X
0973-709X
DOI: 10.7860/JCDR/2018/36923.12060
Popis: Introduction: Thrombophilia is a multi-factorial hypercoagulability disorder. The predisposing factors may be inherited, acquired or both. Factor V Leiden prothrombin 20210G>A mutations are the most common inherited factors. Aim: This study was aimed to investigate the prevalence of Factor V Leiden (1691G>A) and prothrombin 20210G>A mutations in Deep Vein Thrombosis (DVT) patients and to investigate the role of non-genetic risk factors in the manifestation of DVT. Materials and Methods: A total of 192 Sudanese subjects were examined, including 100 DVT patients and 92 healthy controls. Demographic and clinical data were collected using a specific questionnaire. Citrated blood samples of patients and controls were used for coagulation assays, and DNAs isolated from Ethylenediaminetetraacetic Acid (EDTA)-blood samples were used for the detection of Factor V Leiden and prothrombin 20210G>A mutations using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCRRFLP) analysis. Results: Out of all the subjects, none of the 192 subjects carried the Factor V Leiden or prothrombin 20210G>A mutations. No significant differences were detected in the prevalence of DVT between males and females. The prothrombin fragment 1+2, Prothrombin Time (PT), Activated Partial Thromboplastin Time (APTT), and D-dimer levels were significantly elevated in DVT patients than in healthy controls (pA mutations were not accountable for DVT in the Sudanese population examined in this study.
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