Complex Spectroscopic Study for Fusarium Genus Fungi Infection Diagnostics of 'Zalp' Cultivar Oat

Autor: Dmitrii Pankin, Anastasia Povolotckaia, Alexey Kalinichev, Alexey Povolotskiy, Evgenii Borisov, Maksim Moskovskiy, Anatoly Gulyaev, Aleksandr Lavrov, Andrey Izmailov
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Agronomy, Vol 11, Iss 12, p 2402 (2021)
Druh dokumentu: article
ISSN: 2073-4395
DOI: 10.3390/agronomy11122402
Popis: At present, one of the critical problems in agriculture is the identification of cereals, including oats, infected by Fusarium spp. genus fungi. Timely diagnostics can prevent the further disease spread and help to identify the already stored infected grains. In this regard, the aim of this work is to develop the spectroscopic approaches that determine the infected grains. As an object of the investigation the “Zalp” cultivar oat, both healthy and infected grains of the 2020 harvest were chosen. The spectroscopic diagnostics included FTIR in the mid-IR region, Raman, and luminescence methods. Combination of chemometric tools with FTIR and Raman spectroscopy allowed obtaining approaches based on identified characteristic spectral features which may be used as infection markers. These approaches make it possible to detect the infection on the grain husk. The carotenoid type fungi pigment was identified within the resonance conditions of Raman scattering excitation. The luminescence study of infected oat husk revealed the presence of characteristic chlorophyll α peak which is absent in healthy grain husk.
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