| Autor: |
Yang Chen, Xiang-Zhen Han, Wei Wang, Ren-Tao Zhao, Xiao Li |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Bangladesh Journal of Pharmacology, Vol 9, Iss 3 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
1991-0088 |
| DOI: |
10.3329/bjp.v9i3.19568 |
| Popis: |
Human osteosarcoma cells were treated with withaferin A and the antiproliferative activity was evaluated by the 3-(4, 5 dimethyl-thiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assays. Real-time RT-PCR and western blot were used to investigate whether the down-regulation of Notch-1 contributes to withaferin A-induced inhibition of proliferation in osteosar-coma cells. The results showed that withaferin A caused marked inhibition of osteosarcoma cell growth and G2?M phase cell cycle arrest. This was associated with concomitant attenuation of Notch-1 and down-regulation of its downstream genes, such as matrix metalloproteinases. These results suggest that antitumor activity of withaferin A is mediated through a novel mechanism involving inactivation of the Notch-1 signalling pathway. Our data provide the first evidence that the down regulation of Notch-1 by withaferin A may be an effective approach for the treatment of osteosarcoma. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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